|
|
|
| Effects of xenogeneic adipose-derived stem cell transplantation on acute-on-chronic liver failure |
| Wei Zhu, Xiao-Lei Shi, Jiang-Qiang Xiao, Guang-Xiang Gu, Yi-Tao Ding and Zheng-Liang Ma |
Nanjing, China
Author Affiliations: The Combination of Chinese Medicine with Western Medicine Gulou Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210008, China (Zhu W); Department of Anesthesiology (Zhu W and Ma ZL); Department of Hepatobiliary Surgery (Shi XL, Xiao JQ and Ding YT), Affiliated Drum-Tower Hospital, Nanjing University Medical School, Nanjing 210008, China; Jiangsu Province's Key Medical Center for Hepatobiliary Disease, Nanjing 210008, China (Shi XL, Xiao JQ, Gu GX and Ding YT)
Corresponding Author: Zheng-Liang Ma, MD, Department of Anesthe-siology, Affiliated Drum-Tower Hospital, Nanjing University Medical School, Nanjing 210008, China (Tel: 86-25-83105502; Fax: 86-25-83317016; Email: mazhengliang1964@yahoo.com.cn) |
|
|
|
|
Abstract BACKGROUND: Adipose-derived stem cells (ADSCs) are particularly attractive in future clinical applications of stem cell-based therapy for acute-on-chronic liver failure (ACLF). This study was undertaken to evaluate the therapeutic potential of ADSCs on ACLF.
METHODS: ADSCs isolated from porcine fat tissue were expanded and labeled with BrdU. Rabbit models of ACLF were created by administration of D-Gal following CCl4-induced cirrhosis. One day after administration of D-Gal, rabbits of the ACLF/ADSCs group (n=15) were received ADSCs transplantation, while those in the ACLF/saline group (n=15) were treated with the same volume of saline. Biochemical parameters and histomorphological scoring were evaluated; the distribution and characteristics of transplanted ADSCs as well as the pathology of the liver were examined.
RESULTS: ADSCs transplantation improved the survival rate and the liver function of rabbits with ACLF. Biochemical parameters of the ACLF/ADSCs group were improved compared with those of the ACLF/saline group, and histomorphological scoring of the ACLF/ADSCs group was significantly lower than that of the ACLF/saline group. ADSCs were identified in the periportal region of the liver after cell transplantation.
CONCLUSION: Xenogenic ADSCs have therapeutic efficacy in the ACLF rabbit model.
|
|
|
|
|
|
|
|
|
2013, Vol. 12 
