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| Fourier transform infrared spectroscopy of gallbladder carcinoma cell line |
| Jun-Kai Du, Jing-Sen Shi, Xue-Jun Sun, Jian-Sheng Wang, Yi-Zhuang Xu, Jin-Guang Wu, Yuan-Fu Zhang and Shi-Fu Weng |
Xi'an, China
Author Affiliations: Department of General Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China (Du JK, Shi JS, Sun XJ and Wang JS); The College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China (Xu YZ, Wu JG, Zhang YF and Weng SF)
Corresponding Author: Jing-Sen Shi, Professor, Department of General Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China (Tel: 86-29-85323870; Email: shijingsen@126.com) |
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Abstract BACKGROUND: Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line.
METHODS: The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF2 window were measured with a Nicolet Magna750-Ⅱ FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue.
RESULTS: Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue.
CONCLUSION: The diagnosis of carcinoma tissue by FT-IR spectroscopy has a sufficient cellular basis.
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2009, Vol. 8 
