|
|
|
| Nested RT-PCR in detection of blood AFPmRNA in animal model of human hepatocellular carcinoma |
| Yang Liu, Meng-Chao Wu, Guang-Xiang Qian and Bai-He Zhang |
Shanghai, China
From the Hospital of Eastern Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China (Liu Y, Wu MC, Qian GX and Zhang BH)
Correspondence: Yang Liu, MD, Hospital of Eastern Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433, China (Tel: 86-21-25070842; Fax: 86-21-65562400; Email: liuyang@smmu.edu.cn) |
|
|
|
|
Abstract OBJECTIVES: To detect blood AFPmRNA in the nude mice bearing with human hepatocellular carcinoma (HCC) by using nested reverse transcriptase polymerase chain reaction (nested RT-PCR), and assess its significance in HCC distant metastasis.
METHOD: We detected 20 blood samples from the nude mice bearing with human HCC by nested RT-PCR to find out AFPmRNA.
RESULT: AFPmRNA was detected in 6 blood samples from the nude mice bearing with human HCC (30.0%), in which 4 mice developed distant metastasis.
CONCLUSION: AFPmRNA may be used as an efficient and sensitive marker to detect blood spread of HCC cells. It can predict the occurrence of HCC distant metastasis.
|
|
|
|
|
|
|
|
|
2003, Vol. 2 
