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| A rat model for acute hepatic failure |
| Yong He, Jun Zhou, Ke-Feng Dou and Yong Chen |
Xi’an, China
From the Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China (He Y, Dou KF and Chen Y); and the Department of Pathology, Qindu Hospital, Fourth Military Medical University, Xi’an 710032, China (Zhou J)
Correspondence: Yong He, MD, Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China (Tel: 86-29-3375259; Fax: 86-29-3375261; Email: heyong007@yahoo.com) |
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Abstract OBJECTIVE: To develop a surgical model of acute hepatic failure.
METHODS: Hepatectomy was performed in rats according to the method described by Higgins and Anderson. Ninety percent liver resection (removing the left lateral, median, and right lateral lobes) (n=7) and 95% liver resection leaving only half of the caudate lobe (n=13) were performed. Hypoglycemia was corrected by giving 20% glucose in the drinking water, coupled with repeated intraperitoneal injection of 5% glucose adapted to glycemia. While the survival rate, alanine transaminase (ALT) and bilirubin were observed.
RESULTS: 95% liver resection decreased survival rates of the rats from 86% (90% liver resection) to 23% (P<0.05), and increased bilirubin levels (4.04±2.84 mg/dL vs. 1.25±1.85 mg/dL [90% liver resection]).
CONCLUSION: 95% liver resection is a good rat model for acute hepatic failure.
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2003, Vol. 2 
