|
|
|
| Cytomegalovirus infection in liver transplant recipients |
| Jun Fan, Wei-Hang Ma, Jing Qian, Zhi Chen, Dong-Sheng Huang, Wei-Lin Wang and Shu-Sen Zheng |
From the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Fan J, Ma WH, Qian J, Chen Z, Huang DS, Wang WL and Zheng SS)
Correspondence: Jun Fan, MD (Tel: 86-0571-87236581; Fax: 86-0571-87068731; Email: fjjc@yeh.net) |
|
|
|
|
Abstract Objective: To explore cytomegalovirus (CMV) infection in recipients of liver transplantation (LT).
Methods: 30 recipients of LT were screened for the appearance of CMV infection by using ELISA to test anti-CMV-Ab from serum samples and using immunohistochemistry method to test CMV antigen expression and nested-PCR to amplify CMV-DNA from blood samples.
Results: Four of 243 samples taken from 30 recipients came out positive of anti-CMV IgG and anti-CMV IgM with a positive rate of 100% and 1.6% respectively. 85 samples resulted in CMV antigen expression (35.0%) with the average antigen index being 4.2±3.1/5×104 WBC. Besides, 99 samples were found to be positive by nested-PCR with a positive rate of 40.7%. 61 samples were found to be simultaneously positive in test of CMV antigen and DNA, with a rate of 25.1%.
Conclusions: Infection of CMV is common in recipients of LT. Simultaneous screening of anti-CMV-Ab, CMV-Ag and CMV-DNA after liver transplantation is very important for early diagnosis of CMV infection.
|
|
|
|
|
|
|
|
|
2002, Vol. 1 
