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| Effect of reorganized-human growth hormone on cell cycle kinetics in liver cancer in vitro |
| Xin-Yang He, Ge-Liang Xu, Rong-Nan Xu and Zhi-Min Ze |
From the Department of Surgery, Anhui Provincial Hospital, Hefei 230001, China (He XY, XU GL, Xu RN and Ze ZM)
Correspondence: Xin-Yang He, MD (Tel: 86-551-2676 869; Fax: 86-551-5611788; Email: xugelian@mail.hf.ah.cn) |
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Abstract Objective: To study the effect of reorganized-human growth hormone (r-hGH) on cycle kinetics and apoptosis of liver cancer cells or 7402 cells.
Methods: Liver cancer cells were cultured for 24 hours with r-hGH at different concentrations with or without cisplatin (DDP). Cells undergoing apoptosis and differentiation were determined by flow cytometry (FCM).
Results: Comparison of the results in culture with and without r-hGH showed that the percentage of cells in G0-G1 phase dropped (P<0.05), whereas in S phase increased (P<0.05). Adding of r-hGH and DDP to the culture medium increased the apoptosis of liver cancer cells more significantly than adding DDP only (P<0.05).
Conclusions: Liver cancer cells might express the hGH receptor. In vitro r-hGH might induce the differentiation of liver cancer cells, stimulate the combination of DNA, and reduce the cells in G0-G1 phases. These improve the sensibility of tumors to the special-staged chemical treatment. Chemotherapy together with r-hGH may increase the apoptosis of liver cancer cells.
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2002, Vol. 1 
