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| Experimental study of gene therapy with angiostatin gene in pancreatic cancer |
| Sheng-Li Nie and Shi-Zhen Yuan |
From the Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Medical College, Sun Yat-Sen University, Guangzhou 510120, China (Nie SL and Yuan SZ)
Correspondence: Sheng-Li Nie, PhD (Tel: 86-20-82349599; Email: victorynie@163.com) |
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Abstract Objective: To study the effects of angiostatin(AS) gene mediated by liposome on human pancreatic cancer cell line SW1990.
Methods: Angiostatin gene was cloned into the eukaryotic expression vector pRC/CMV. The recombinant of pRC/CMV-AS was introduced into the pancreatic cancer cell line, SW1990. The mechanism of anti-tumor was studied and tested.
Results: The eukaryotic expression vector pRC/CMV-AS was identified by the restriction digest. pRC/CMV-AS was stably integrated into the target cells and expressed by Western blot and drug-sensitivity tests, and inhibited the vascular endothelial cells proliferation in vitro. In addition, the effects of the angiostatin vector on reducing the volume of tumors implanted in nude mouse models were also noted.
Conclusion: This study demonstrated that the recombinant pRC/CMV-AS mediated by liposome may play a potential role in the treatment of pancreatic cancer in the future.
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2002, Vol. 1 
