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| Role of mitogen activated protein kinase cascade pathway and anti-stress response in liver transplantation |
| Nian-Qiao Gong, Jian-Sheng Xiao, Jin-Sheng Zeng, Guo-Xun Li, Hui Guo, Wei-Jie Zhang and Qi-Fa Ye |
Wuhan, China
From the Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China (Gong NQ, Xiao JS, Zeng JS, Li GX, Guo H, Zhang WJ and Ye QF)
Correspondence: Nian-Qiao Gong, MD (Tel: 86-27-8366 3409; Fax: 86-27-83808080; Email: nqgong@tjh.tjmu.edu.cn) |
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Abstract OBJECTIVE: To explore the changes of mitogen activated protein kinase (MAPK) cascade pathway and anti-stress response of hepatocytes after liver transplantation.
METHODS: Ten normal liver specimens and 18 punctured donor liver specimens were divided into 3 groups: A (control∶10), B (no rejection∶10) and C (acute rejection∶8). MAPK, Ras, Jun and heat shock protein 70 (HSP70) were tested immunohistochemically while Ras and HSP70 were tested by in situ hybridization. All sections were subjected to image analysis.
RESULTS: Protein expressions of MAPK, Ras and Jun were increased by an ascending order of groups A, B and C. The protein expression of HSP70 was the highest in group B but lower in group C. Expressions of Ras and HSP70 mRNA were consistent with those of protein.
CONCLUSIONS: The changes of the MAPK cascade pathway and anti-stress response of hepatocytes after liver transplantation are one of regulation mechanisms for protecting the hepatocytes from damage after liver transplantation. This mechanism is active to support individual survival.
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2003, Vol. 2 
