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| Expression of AFPmRNA in blood of patients with recurrent hepatocellular carcinoma |
| Yang Liu, Bai-He Zhang, Guang-Xiang Qian, Han Chen and Meng-Chao Wu |
Shanghai, China
From the Eastern Hospital of Hepatobiliary Surgery, Se-cond Military Medical University, Shanghai 200433, China (Liu Y, Zhang BH, Qian GX, Chen H and Wu MC)
Correspondence: Yang Liu, MD (Tel: 86-21-25070842; Fax: 86-21-65562400; Email: liuyang@smmu.edu.cn) |
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Abstract OBJECTIVES: To detect the expression of human alpha-fetoprotein mRNA (AFPmRNA) in peripheral blood (PBL) of recurrent hepatocellular carcinoma (HCC) by using nested reverse transcriptase polymerase chain reaction (nested RT-PCR) and to discuss relations between AFPmRNA and recurrent HCC.
METHODS: Five ml peripheral blood samples were obtained from 19 patients with recurrent HCC. To identify HCC cells in PBL, liver-specific AFPmRNA was amplified from the total RNA extracted from the whole blood by nested RT-PCR.
RESULTS: Seven patients (36.8%) with recurrent HCC were detected AFPmRNA, and 12 patients with recurrent HCC were AFPmRNA negative (63.2%). There was no significant relation between patients with AFPmRNA positive and AFPmRNA negative (P>0.05) in judging the origin of recurrent HCC.
CONCLUSION: AFPmRNA may not be used as a marker of origin of recurrent HCC.
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2003, Vol. 2 
