|
|
|
| Establishment of hepatocarcinoma cell line transfected by the B7 gene and its Biocharacteristics |
| You-Zhu Li, Quan Wang and Yan-Fang Jiang |
Changchun, China
From the First Clinical Hospital, Jilin University, Changchun 130021, China (Li YZ, Wang Q and Jiang YF)
Correspondence: You-Zhu Li, MD (Tel: 86-431-5612811; Fax: 86-431-5654528; Email: leeyz@public.cc.jl.cn) |
|
|
|
|
Abstract OBJECTIVE: To study the function of costimulation sign in tumor immunology and construct the new cell line B7+ Smmc7721.
METHODS: The B7 gene was transfected into the hepatocarcinoma cell Smmc7721 by liposma. Polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR) were applied to test the result. MTT colorimetric assay was used to value the killing effect of lymphokine activated killer cells (LAK) activated by IL-2 on the transfected cell line and the original line.
RESULT: B7+ Smmc7721 was improved to be steadily expressed B7 molecule and LAK cells could more effectively act on the B7+ Smmc7721 cells.
CONCLUSION: The B7 gene can be transfected to hepatocarcinoma cells and can be expressed steadily in vitro, thus increasing the efficiency of LAK cells activated by IL-2 on them.
|
|
|
|
|
|
|
|
|
2003, Vol. 2 
