|
|
|
| Development of an enzyme immune assay for detecting M2 autoantibodies specific for primary biliary cirrhosis |
| Xiao-Hua Jiang, Xiao-Yun Fang, Ren-Qian Zhong, Xiu-Ping Wang, Yin Hu and Xian-Tao Kong |
Shanghai, China
From the Department of Laboratory Medicine, 85th Military Hospital, Shanghai 200052, China (Jiang XH, Fang XY and Wang XP); People’s Liberation Army Clinical Immunology Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China (Zhong RQ and Kong XT); Shanghai University of Engineering Science, Shanghai 200335, China (Hu Y)
Correspondence: Xiao-Hua Jiang, MD (Tel: 86-21-62528805; Email: jhlulu@citiz.net) |
|
|
|
|
Abstract OBJECTIVE: To develope a new enzyme immune assay (ELISA) for detection of M2 antibody specific for primary biliary cirrhosis (PBC) by using a triple hybrid clone as antigen, which coexpresses the three immunodominant lipoyl domains of PDC-E2, BCOADC-E2 and OGDC-E2 from human sources.
METHODS: After expressing autoantigens of PBC in prokaryote by constructing recombinant expressive plasmid successfully, the fusion protein was purified by affinity chromatography. The sera of 17 PBC patients were examined. As controls, the sera of 167 non-PBC patients and the sera of 1225 normal controls aged under 28 were examined.
RESULTS: None of the sera from the non-PBC patients or the normal controls was positive for anti-M2 shown by the new ELISA. However, the positivity rate for anti-M2 in the PBC patients was 100% (17/17), as shown by the new ELISA.
CONCLUSION: The detection system with a good sensitivity and specificity may be used as a powerful method for the diagnosis of PBC.
|
|
|
|
|
|
|
|
|
2003, Vol. 2 
