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| Identification of a gene engineering antibody against cystic echinococcosis in liver |
| Xin-Hua Chen, Hao Wen, Yao-Xin Zhang, Xiao-Hui Feng, Xiao-Mei Lu and Dong Ma |
Urumqi, China
From the Xinjiang Hydatid Clinical Research Institute (Chen XH, Wen H, Feng XH, Lu XM and Ma D), and the Department of Infectious Diseases (Zhang YX), First Teaching Hospital, Xinjiang Medical University, Urumqi 830054, China
Correspondence: Hao Wen, MD, PhD, Xinjiang Hydatid Clinical Research Institute, No.1 Liyushan Road, Urumqi 830054, China (Tel: 86-991-4362844; Fax: 86-991-432 4139; Email: wenhao2002@hotmail.com)
The project was supported by a grant from the National Natural Science Fundation of China (No. 39860078) and Xinjiang Natural Science Fundation China (No. 200221 101). |
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Abstract OBJECTIVE: To identify a gene engineering antibody against cystic echinococcosis in liver.
METHODS: A single chain of variable fragment of human antibodies (ScFvs) was selected from the library by using affinity selection technique with the recombinant antigen on solid surface. The positive clones were demonstrated by ELISA and their DNA sequences were also determined.
RESULTS: The DNA sequence data showed that the antibody gene is composed of 768bp. In addition, a specific combination capacity with recombinant Echinococcus granulosus antigen B (r-EgB) was demonstrated by ELISA.
CONCLUSION: The obtained gene engineering antibody against r-EgB may have potential implications in immunological treatment and drug targeting delivery.
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2003, Vol. 2 
