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Bile anaerobic bacteria detection andantibiotic susceptibility in patients with gallstone |
Yun Lu, Ting-Hai Xiang, Jing-Sen Shi and Bing-Yuan Zhang |
Qingdao, China
From the Department of Hepatobiliary Vascular Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China (Lu Y, Xiang TH and Zhang BY); Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi’an Jiaotong University, Xi’an 710061, China (Shi JS)
Correspondence: Yun Lu, MD, Department of Hepatobiliary Vascular Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China (Tel: 86-532-2911369; Fax: 86-532-2911999; Email: cloudylucn@yahoo.com) |
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Abstract OBJECTIVE: To detect bile anaerobic bacteria and antibiotic susceptibility in 59 patients with gallstones who had had cholecystectomy.
METHODS: BACT/ALERT 120 microbe detection system and SCEPTOR microbe detection system were used to detect bile anaerobic bacteria, antibiotic susceptibility.
RESULTS: The ratio of anaerobic bacteria to the patients examined was 52.5% (31/59). Obligate anaerobe bile culture showed positive results in 4 patients. B. fragilis (37.8%) was the major type of anaerobic bacteria in bile. Most (81.8%) of anaerobic bacteria were sensitive to metronidazole, and imipenem was suitable for β-lactamase bacteria.
CONCLUSIONS: Culture of anaerobic bacteria in logarithmic phase can improve the positive rate of the culture. There are some relations between anaerobic infection and gallstone formation.
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