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Aberration of X chromosome in liver neoplasm detected by fluorescence in situ hybridization |
Jun Liu, Zhan-Min Wang, Shu-Fang Zhen, Xiao-Peng Wu, Dao-Xin Ma, Zhao-Hui Li, Bo Liu, Zhi-Lun Zhao and Yang Ke |
Jinan, China
Author Affiliations: Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, China (Liu J, Wang ZM, Wu XP, Ma DX, Li ZH, Liu B and Zhao ZL); Clinical Tumor Institute of Beijing University, Beijing 100014, China (Zhen SF and Ke Y)
Corresponding Author: Jun Liu, MD, PhD, Department of General Surgery, Qilu Hospital, Shandong University, Jinan 250012, China (Tel: 86-531-6921941 ext 5426; Fax: 86-531-6927544; Email: dr_ liujun@hotmail.com) |
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Abstract BACKGROUND: A diverse range of cytogenetic alterations of autosomal chromosomes has been reported to date. However, few studies have addressed the abnormalities of X chromosome in hepatocellular carcinoma (HCC) except sporadic reports on the deletion of band F1 in X chromosome, and the clonal analysis of methylation pattern of the X chromosome-linked human androgen receptor gene. Identification of specific X chromosome alterations during the course of neoplastic development would be essential to defining the genetic basis of HCC. Therefore, we studied the regularity of aberration of X chromosome in liver cancer.
METHODS: Hepatocarcinoma cellular lines and tumor tissues were detected respectively through DNA probes of X chromosome after fluorescence in situ hybridization (FISH).
RESULTS: Increased copies of X chromosome were observed in all samples, and four signals of hybridization were of the major type.
CONCLUSIONS: Increased copy number of X chromosome frequently occur in liver cancer. The relationship between copy number of X chromosome and liver cancer genesis needs further investigation. This study is the first of its kind determining the copy number of X chromosome in liver cancer by using FISH.
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