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| Inhibition of hepatitis C virus-transfected cholangiocarcinoma by antisense oligodeoxynucleotide in nude mice |
| Xiao-Fang Liu, Xian-Ting Zhou and Sheng-Quan Zou |
Yantai, China
Author Affiliations: Department of Hepatobiliary Surgery, Affiliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China (Liu XF and Zhou XT); Department of General Surgery, Tongji Medical Hospital, Wuhan 430030, China (Zou SQ)
Corresponding Author: Xiao-Fang Liu, MD, Department of Hepatobiliary Surgery, Affiliated Yantai Yuhuangding Hospital, Qingdao University Medical College, Yantai 264000, China (Tel: 86-535-6691999; Fax: 86-535-6240341; Email: liu634@263.net) |
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Abstract BACKGROUND: The inhibitory effect of antisense oligodeoxynucleotide (asODN) on the replication and expression of hepatitis C virus (HCV) in vitro is not well elucidated. This study was to assess the effect of asODN on HCV in cholangiocarcinoma.
METHODS: The QBC939 cells transfected by a recombinant HCV containing HCV core gene cloned in vector of PBK-CMV (PBK-HCVC) were treated by 14-mers phosphorothioate ODN complementary to the HCV core genomic region. The variation of HCVmRNA level was detected by RT-PCR. Moreover, the inhibitory effect of asODN was observed in nude mice.
RESULTS: HCVmRNA was detected in transfected-QBC939 cells. The 14-mers complementary phosphorothioate ODN showed effective inhibition on HCVmRNA and unexpression HCVmRNA at 6 μmol/L. The tumorigenicity of the transfected-QBC939 cells incubated with asODN in nude mice was greatly inhibited.
CONCLUSION: The results suggest a potential therapy of asODN for HCV infected cholangiocarcinoma.
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2004, Vol. 3 
