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Inhibitory effects of antisense oligodeoxynucleotide on expression of vascular endothelia growth factor by human hepatocarcinoma cells |
Meng-Biao Qiu, Ji-Xiang Zhang, Liang-Ming Liu, Bang-Dong Gong, Bo-Lin Wu, Shui-Shan Zhu and Yi Wen |
Nanchang, China
Author Affiliations: Molecular Center, Second Affiliated Hospital of Jiangxi Medical College, Nanchang 330006, China (Qiu MB, Zhang JX, Liu LM, Gong BD, Wu BL, Zhu SS and Wen Y)
Corresponding Author: Ji-Xiang Zhang, MD, Molecular Center, Second Affiliated Hospital of Jiangxi Medical College, Nanchang 330006, China (Tel: 86-791-6291870; Email: jixiangz@163.net) |
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Abstract BACKGROUND: It is widely recognized that the growth of solid tumor depends on angiogenesis. Vascular endothelia growth factor (VEGF) is an endothelial cell-specific mitogen that promotes angiogenesis in solid tumor. Inhibition of angiogenesis is considered a promising approach for cancer therapy, and treatments including administration of antisense drugs and RNA interference for the VEGF gene are geared to the suppression of tumor angiogenesis.
METHODS: As a new approach for gene therapy of hepatocellular carcinoma (HCC), four groups of antisense oligodeoxynucleotide (ASODN) (A-Cap, A-AUG, A-UGA and A-Exon-3) were used to block the expression of VEGF, then VEGF mRNA and protein were detected by RT-PCR and Western blot.
RESULTS:
After treatment with ASODN, the relative VEGF mRNA levels of A-Cap, A-AUG, A-UGA, and A-Exon-3 were decreased significantly to (32±9)%, (63±1)%, (86±3)%, and (70±5)%, respectively(F=64.18, P<0.001). The relative VEGF protein levels of A-Cap, A-AUG, A-UGA and A-Exon-3 were decreased significantly to (41±5)%, (59±3)%, (88±7)%, and (79±9)% respectively (F=60.64, P<0.001).
CONCLUSIONS: Among the four ASODNs, the ASODN for Cap structure showed the strongest inhibitory effect and that for A-UGA, the least (P<0.05 ). The inhibitory effect of ASODN on the expression of VEGF proteins was similar to that of VEGF mRNA expression.
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Cite this article: |
Qiu MB,
Zhang JX,
Liu LM,
et al.
Inhibitory effects of antisense oligodeoxynucleotide on expression of vascular endothelia growth factor by human hepatocarcinoma cells.
Hepatobiliary Pancreat Dis Int
2004;
3(4):
552-557. DOI:
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URL: |
http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2004/V3/I4/552 |
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