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Abnormal expression of hepatoma-derived γ-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes |
Deng-Fu Yao, Zhi-Zhen Dong, Deng-Bing Yao, Xin-Hua Wu, Wei Wu, Li-Wei Qiu, Hong-Mei Wang and Xian-Yong Meng |
Nantong, China
Author Affiliations: Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China (Yao DF, Dong ZZ, Wu XH, Wu W, Qiu LW, Wang HM and Meng XY); Institute of Neurosciences, Nantong University, Nantong 226001, China (Yao DB)
Corresponding Author: Deng-Fu Yao, MD, PhD, Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China (Tel: 86-513-5861539; Fax: 86-513-5052523; Email: dfyrcmb@yahoo.com) |
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Abstract BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase (GGT) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kinetic expression and the early alterations of GGT in the development of hepatoma remain unclear. In this study, we investigated the expression and the alterations of GGT multiple molecular forms in hepatotumorigenesis.
METHODS: The expression of GGT in a chemically induced hepatocarcinogenesis model was examined by giving 0.05% of 2-fluoenylacetamide in diet for 12 weeks. The expression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar-chain heterogeneity were investigated at the different stages of rat hepatoma development.
RESULTS: Pathological examination and biochemical analysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and membrane-combined GGT were significantly higher (P<0.05) in experimental groups than those in control group, respectively. A highly positive correlation was found between total GGT activities and total RNA levels (r=0.90, P<0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly during the development of rat hepatoma. GGT multiple molecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma.
CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer.
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Cite this article: |
Yao DF,
Dong ZZ,
Yao DB,
et al.
Abnormal expression of hepatoma-derived γ-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes.
Hepatobiliary Pancreat Dis Int
2004;
3(4):
564-570. DOI:
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URL: |
http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2004/V3/I4/564 |
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