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Interleukin-10 promoter polymorphisms in patients with hepatitis B virus infection or hepatocellular carcinomain Chinese Han ethnic population |
Juan Wang, Hong Ni, Li Chen and Wen-Qin Song |
Tianjin, China
Author Affiliations: College of Life Sciences, Nankai University, Tianjin 300071, China (Wang J, Ni H, Chen L and Song WQ) and College of Life Sciences, Shenzhen University, Shenzhen 518060, China (Wang J)
Corresponding Author: Wen-Qin Song, MD, College of Life Sciences, Nankai University, Tianjin 300071, China (Tel: 86-22-23508241; Fax: 86-22-24397010; Email: songwq@eyou.com) |
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Abstract BACKGROUND: Since single nucleotide polymorphisms (SNPs) can serve as gene markers, polymorphism profiles may help scientists to identify the full collection of genes that contribute to the development of complex diseases such as cancer. The distribution of interleukin-10 (IL-10) promoter polymorphisms in Chinese Han ethnic patients with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC) was investigated in this study.
METHODS: The polymorphisms of IL-10 promoter region were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing. Sixty-six health controls, 42 patients with HBV infection, 30 HCC patients, and cell line SMMC-7721 were examined this way.
RESULTS: Polymorphisms of T/C or T/N on -872 site occurred frequently in Han ethnic population. Polymorphisms were detected in HBV and HCC patients and cell line SMMC-7721. The hotspot among the polymorphisms was inserting base A between -1058 and -1057.
CONCLUSION: Polymorphisms of IL-10 promoter in HBV and HCC patients may be associated with HBV infection and HCC development.
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Cite this article: |
Wang J,
Ni H,
Chen L,
et al.
Interleukin-10 promoter polymorphisms in patients with hepatitis B virus infection or hepatocellular carcinomain Chinese Han ethnic population.
Hepatobiliary Pancreat Dis Int
2006;
5(1):
60-64. DOI:
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URL: |
http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2006/V5/I1/60 |
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