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| Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma |
| Wei-Jun Jiang, Xiao-Bo Man, Liang Tang, Hong-Yin Song, Shen-Jing Li, Guo-Jun Cai, Xiu-Hua Qiu and He-Ping Hu |
Shanghai, China
Author Affiliations: Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai 200050, China (Jiang WJ and Song HY); Department of Gastroenterology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China (Man XB, Tang L, Li SJ, Qiu XH and Hu HP); and Department of Pharmacology, Second Military Medical University, Shanghai 200433, China (Cai GJ)
Corresponding Author: He-Ping Hu, MD, Department of Gastroenterology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China (Tel: 86-21-25070785; Fax: 86-21-25070785; Email: hp-hu@medmail.com.cn) |
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Abstract Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinomaBACKGROUND: OCI-5, the rat homologene of human glypican 3 (GPC3), is confirmed upregulated in hepatocellular carcinoma (HCC). The present study was undertaken to detect gene expression change of OCI-5 during occurrence and progression of rat HCC.
METHODS: Male Sprague-Dawley rats were given diethylnitrosamine (DENA) to induce HCC. Three DENA-induced rats and one control rat were sacrificed every week for 18 weeks during the development of HCC. Tissues specimens were snap-frozen in liquid nitrogen and total RNA was isolated. Sk-Hep1 cells were treated with DENA at different concentrations. The gene expression levels of OCI-5 and GPC3 were detected with the RT-PCR method.
RESULTS: OCI-5 was not expressed in normal rat liver tissues. When HCC occurred and aggravated, OCI-5 expression was gradually elevated to a very high level. GPC3 was not expressed in the DENA-treated Sk-Hep1 cells.
CONCLUSIONS: OCI-5 was not expressed in normal rat liver tissues but in rat HCC tissues. High-expression of OCI-5 in DENA-induced rat HCC model was the gene expression change of HCC not the DENA-induced gene expression. The expression level of OCI-5 was not only elevated in rat HCC but also gradually along the occurrence and progression of HCC, indicating that GPC3 might serve as a sensitive marker of early stage HCC.
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2006, Vol. 5 
