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Effect of L-arginine on calcium in hepatic mitochondrion in rats with obstructive jaundice |
Mu-Sheng Lin, Hui-Lai Miao, Xiao-Guang Gong and Shi-Ting Bao |
Zhanjiang, China
Author Affiliations: Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, China (Lin MS, Miao HL, Gong XG and Bao ST)
Corresponding Author: Mu-Sheng Lin, MD, Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, China (Tel: 86-759-2388500; Email: linmusheng@gdmc.edu.cn) |
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Abstract BACKGROUND: There is much debate over the regulation of mitochondrial calcium overload and reducing the impairment of energy metabolism in hepatic cells. It has not been reported whether L-arginine (L-Arg) can affect hepatic mitochondrial calcium overload. This study was undertaken to investigate the protective effect of L-Arg on Ca2+ handling of hepatic mitochondrion in rats with obstructive jaundice and to clarify its possible mechanism.
METHODS: Seventy-two male SD rats were randomly divided into 3 groups: sham operation+normal saline group (SO group), common bile duct ligation+normal saline group (BDL group), and common bile duct ligation+ L-Arg group (L-Arg group). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and Ca2+ in rat hepatic mitochondrion were examined at the 7th, 14th and 21st day after operation.
RESULTS: The Ca2+ and MDA levels of hepatic mitochondrion increased significantly but their SOD content decreased markedly at each time point in the BDL group. Except at the 21st day, the Ca2+ and MDA, contents of hepatic mitochondrion were significantly lower, and SOD concentrations were higher in the L-Arg group than those in the BDL group at the 7th and 14th day (P<0.01).
CONCLUSION: L-Arg has a protective effect on mitochondrion in the early and mid stages of obstructive jaundice.
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