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| Donor denervation and elimination of Kupffer cells affect expression of P-selectin and ICAM-1 in liver graft |
| Hong Zhu, Yong-Zhang Guo, Jie Zhang, Ming-Dao Hu, Ji-Hong Tang, Da-Guang Tian, Yong-Jun A, Feng Sun and Xiao-Ping Wei |
Kunming, China
Author Affiliations: First Division of Hepatobiliary Department, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China (Zhu H, Guo YZ, Zhang J, Hu MD, Tang JH, Tian DG, A YJ, Sun F and Wei XP)
Corresponding Author: Hong Zhu, MD, First Division of Hepatobiliary Department, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China (Tel: 86-871-5351281ext2681; Email: zhhong 519@163.com) |
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Abstract BACKGROUND: The non-function and dysfunction of primary liver graft likely involves dependence on Kupffer cells and hepatic innervation. The present experiment was designed to study the expression of P-selectin and intercellular adhesion molecule-1 (ICAM-1) mRNA in liver graft and to elucidate the role of Kupffer cells and the sympathetic nerve of the liver in down-regulating this expression.
METHODS: Donor rats were given hexamethonium, a sympathetic ganglionic blocking agent, and/or gadolinium chloride, an inhibitor of Kupffer cells. Then the changes of graft P-selectin and ICAM-1 mRNA expression were measured after liver transplantation.
RESULTS: The expressions of P-selectin and ICAM-1 mRNA were increased after liver transplantation, and down-regulated by liver denervation and elimination of Kupffer cells.
CONCLUSIONS: Live donor denervation and elimination of Kupffer cells down-regulated the expressions of P-selectin and ICAM-1 mRNA in grafts. This may decrease graft ischemia/reperfusion injury.
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2007, Vol. 6 
