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The potential molecular mechanism of overexpression of uPA, IL-8, MMP-7 and MMP-9 induced by TRAIL in pancreatic cancer cell |
Dong-Hui Zhou, Anna Trauzold, Christian Röder, Gang Pan, Cheng Zheng and Holger Kalthoff |
Hangzhou, China
Author Affiliations: Department of Oncology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Zhou DH, Pan G and Zheng C); and Molecular Oncology Research Laboratory, Clinic for General and Thoracic Surgery, Christian-Albrechts-University, 24105 Kiel, Germany (Trauzold A, Röder C and Kalthoff H)
Corresponding Author: Dong-Hui Zhou, MD, Department of Oncology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Tel: 86-571-87236878; Fax: 86-571-86027898; Email: zdh19838@hotmail.com) |
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Abstract BACKGROUND: TNF-related apoptosis-inducing ligand (TRAIL) is a death ligand of the TNF-superfamily that has been implicated in inducing apoptosis in some tumor cells. The purpose of this study was to find out if TRAIL could induce the expression of uPA, IL-8, MMP-7 and MMP-9.and to explore the corresponding potential signaling transduction pathway in pancreatic cancer cells.
METHODS: Colo357wt, Panc89 and PancTuⅠ cell lines were stimulated by TRAIL (100 ng/ml). Crystal violet cell vitality assay was used to check the sensitivity to TRAIL-induced apoptosis. Real-time RT-PCR tested the expression of uPA, IL-8, MMP-7 and MMP-9.
RESULTS: TRAIL can stimulate the expression of uPA, IL-8, MMP-7 and MMP-9 in pancreatic cancer cell lines, especially in Colo357wt. The members of caspases, MEK1/2, PKC, and NF-κB are involved in TRAIL-induced expression of uPA, IL-8, MMP-7 and MMP-9. Furthermore, caspases play a different role in Colo357wt, Panc89 and PancTuⅠ.
CONCLUSIONS: TRAIL-treatment may result in the enhancement of invasion involving the signaling pathways of caspases, MEK1/2, PKC and NF-κB, in pancreatic cancer cells. It points to the necessity to carefully evaluate in vivo side effects of TRAIL.
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Cite this article: |
Zhou DH,
Trauzold A,
R?der C,
et al.
The potential molecular mechanism of overexpression of uPA, IL-8, MMP-7 and MMP-9 induced by TRAIL in pancreatic cancer cell.
Hepatobiliary Pancreat Dis Int
2008;
7(2):
201-209. DOI:
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URL: |
http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2008/V7/I2/201 |
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