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| Influence of DNA methyltransferase 3b on FHIT expression and DNA methylation of the FHIT promoter region in hepatoma SMMC-7721 cells |
| Jia-Xiang Wang, Yong-Gan Zhang and Long-Shuan Zhao |
Zhengzhou, China
Author Affiliations: Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China (Wang JX, Zhang YG and Zhao LS)
Corresponding Author: Jia-Xiang Wang, MD, Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China (Tel: 86-371-66963082; Email: wjiaxiang@zzu.edu.cn) |
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Abstract BACKGROUND: Alterations in DNA methylation occur during the pathogenesis of human tumors. In this study, we investigated the influence of DNA methyltransferase 3b (DNMT3b) on fragile histidine trial (FHIT) expression and on DNA methylation of the FHIT promoter region in the hepatoma cell line SMMC-7721.
METHODS: DNMT3b siRNA was used to down-regulate DNMT3b expression. DNMT3b and FHIT proteins were determined by Western blotting. Methylation-specific PCR was used to analyze the methylation status of the FHIT gene.
RESULTS: After DNMT3b siRNA transfection, the expression of DNMT3b was inhibited in SMMC-7721 cells, and the expression of FHIT was significantly higher than that in the control group. There was no significant difference in methylation status between the DNMT3b siRNA transfected cells and control cells.
CONCLUSION: DNMT3b may play an important role in regulation of FHIT expression in hepatoma SMMC-7721 cells, but not through methylation of the FHIT promoter.
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| Cite this article: |
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Wang JX,
Zhang YG,
Zhao LS.
Influence of DNA methyltransferase 3b on FHIT expression and DNA methylation of the FHIT promoter region in hepatoma SMMC-7721 cells.
Hepatobiliary Pancreat Dis Int
2009;
8(3):
273-277. DOI:
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| URL: |
| http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2009/V8/I3/273 |
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2009, Vol. 8 
