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| Expression pattern of mda-7/IL-24 receptors in liver cancer cell lines |
| Hong Zhu and Zhi-Bin Yang |
Kunming, China
Author Affiliations: Division of Hepatobiliary, Second Affiliated Hospital, Kunming Medical College, Kunming 650101, China (Zhu H); Abdominal Cancer Centre, Third Affiliated Hospital, Kunming Medical College, Kunming 650102, China (Yang ZB)
Corresponding Author: Zhi-Bin Yang, MD, Abdominal Cancer Centre, Third Affiliated Hospital, Kunming Medical College, Kunming 650102, China (Tel: 86-13987123967; Email: yzblbab@vip.sina.com) |
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Abstract BACKGROUND: The mda-7/IL-24 receptor belongs to the type Ⅱ cytokine receptor family, and its two heterodimeric receptors are IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Mda-7/IL-24 receptor expression in liver cancer cell lines has not yet been described. This information may be helpful for further clinical gene therapy.
METHODS: With normal skin total RNA as template, the cDNA sequences of IL-20R1, IL-20R2 and IL-22R were amplified by RT-PCR. Total RNA was extracted from cultured liver cancer cell lines and a normal liver cell line, then detected by northern blotting, and the expression of mda-7/IL-24 receptors was analyzed.
RESULTS: PLC/PRF/5 and SMMC-7721 expressed IL-20R1; BEL-7402, Hep3B, HepG2, and PLC/PRF/5 expressed IL-20R2; and HepG2 and PLC/PRF/5 expressed IL-22R. Only HepG2 expressed the IL-22R/IL-20R2 receptor complex. PLC/PRF/5 completely expressed both heterodimeric receptors. Huh-7, QGY-7701 and WRL-68 did not express the IL-24 receptor.
CONCLUSION: Complete mda-7/IL-24 receptors are seldom expressed in liver cancer cell lines.
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2009, Vol. 8 
