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Profile of hepatocyte apoptosis and bile lakes before and after bile duct decompression in severe obstructive jaundice patients |
Toar JM Lalisang, Raden Sjamsuhidajat, Nurjati C Siregar and Akmal Taher |
Jakarta, Indonesia
Author Affiliations: Department of Surgery (Lalisang TJM and Sjamsuhidajat R) and Department of Anatomical Pathology (Siregar NC), Faculty of Medicine, University of Indonesia; Department of Urology, Cipto Mangunkusumo Hospital (Taher A), Jakarta, Indonesia
Corresponding Author: Toar JM Lalisang, PhD, Digestive Surgery Division, Department of Surgery, University of Indonesia, Cipto Mangunkusumo Hospital Jakarta, Jl. Diponegoro 71, Jakarta 10430, Indonesia (Tel/Fax: +62- 21-3148705; Email: toar@doctor.com) |
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Abstract BACKGROUND: Excessive hepatocyte apoptosis and bile lakes in severe obstructive jaundice might impair liver functions. Although decompression of the bile duct has been reported to improve liver functions in animal studies, the mechanism of obstruction differs from that in humans. This study aimed to determine the profiles of hepatocyte apoptosis and bile lakes following bile duct decompression in patients with severe obstructive jaundice in the clinical setting.
METHODS: We conducted a "before and after study" on severe obstructive jaundice patients as a model of inhibition of the excessive process by bile duct decompression. Specimens of liver biopsies were taken before and after decompression of the bile duct and then stained by terminal deoxynucleotide transferase-mediated dUTP nick end-labeling (TUNEL) to identify hepatocyte apoptosis and by hematoxilin-eosin (HE) to identify bile lakes. All measurements were independently done by 2 observers.
RESULTS: Twenty-one severe obstructive jaundice patients were included. In all patients, excessive hepatocyte apoptosis and bile lakes were apparent. After decompression, the hepatocyte apoptosis index decreased from 53.1 (SD 105) to 11.7 (SD 13.6) (P<0.05), and the bile lakes from 23.6 (SD 14.8) to 10.9 (SD 6.9) (P<0.05).
CONCLUSION: Bile duct decompression improves hepatocyte apoptosis and bile lakes in cases of severe obstructive jaundice, similar to the findings in animal studies.
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Cite this article: |
Lalisang TJM,
Sjamsuhidajat R,
Siregar NC,
et al.
Profile of hepatocyte apoptosis and bile lakes before and after bile duct decompression in severe obstructive jaundice patients.
Hepatobiliary Pancreat Dis Int
2010;
9(5):
520-523. DOI:
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URL: |
http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2010/V9/I5/520 |
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