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Oncolytic adenovirus-mediated MDA-7/IL-24 overexpression enhances antitumor activity in hepatocellular carcinoma cell lines |
Chao-Wen Xiao, Xin-Bo Xue, Hui Zhang, Wei Gao, Yuan Yu, Kun Chen, Jian-Wei Zheng and Cong-Jun Wang |
Shanghai, China
Author Affiliations: Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China (Xiao CW, Xue XB, Yu Y, Chen K and Zheng JW); Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China (Zhang H, Gao W and Wang CJ)
Corresponding Author: Cong-Jun Wang, MD, PhD, Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China (Tel: 86-21-38804518ext2704; Email: wcj902@163.com) |
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Abstract BACKGROUND: Melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL-24) is a novel tumor suppressor gene, which has suppressor activity in a broad spectrum of human cancer cells. We investigated the effect of the replication-competent oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus Ad.IL-24, both expressing human MDA-7/IL-24 on the hepatocellular carcinoma cell lines HepG2, Hep3B, SMMC-7721, HCCLM3, and the normal liver cell line L02.
METHODS: Hepatocellular carcinoma cell lines and the normal liver cell line were infected with SG600-IL24 and Ad.IL-24. The mRNA and protein expression of MDA-7/IL-24 in infected cells was confirmed by RT-PCR, ELISA, and Western blotting. MTT assay was used to investigate the proliferation effect. Hoechst staining and Annexin-V and PI staining were performed to study the MDA-7/IL-24 gene expressed in HCC cell lines and the normal liver cell line. Flow cytometry was used to analyse the cell cycle.
RESULTS: RT-PCR, ELISA and Western blotting confirmed that the exogenous MDA-7/IL-24 gene was highly expressed in cells infected with SG600-IL24. MTT and apoptosis detection indicated that SG600-IL24 induced growth suppression, promoted apoptosis, and blocked cancer cell lines in the G2/M phase in hepatocellular carcinoma cell lines but not in the normal liver cell line.
CONCLUSIONS: SG600-IL24 selectively induces growth suppression and apoptosis in hepatocellular carcinoma cell lines in vitro but not in the normal liver cell line L02. Compared with Ad.IL-24, SG600-IL24 dramatically enhances antitumor activity in hepatocellular carcinoma cell lines.
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Cite this article: |
Xiao CW,
Xue XB,
Zhang H,
et al.
Oncolytic adenovirus-mediated MDA-7/IL-24 overexpression enhances antitumor activity in hepatocellular carcinoma cell lines.
Hepatobiliary Pancreat Dis Int
2010;
9(6):
615-621. DOI:
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URL: |
http://dx.doi.org/ OR http://www.hbpdint.com/EN/Y2010/V9/I6/615 |
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