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| Polymorphisms of CCL3L1/CCR5 genes and recurrence of hepatitis B in liver transplant recipients |
| Hong Li, Hai-Yang Xie, Lin Zhou, Wei-Lin Wang, Ting-Bo Liang, Min Zhang and Shu-Sen Zheng |
Hangzhou, China
Author Affiliations: Key Laboratory of Combined Multi-Organ Transplan-tation, Ministry of Public Health (Li H, Xie HY, Zhou L and Zheng SS), and Division of Hepatobiliary Pancreatic Surgery (Wang WL, Liang TB, Zhang M and Zheng SS), First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
Corresponding Author: Shu-Sen Zheng, MD, PhD, FACS, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Tel: 86-571-87236570; Fax: 86-571-87236628; Email: shusenzheng@zju.edu.cn) |
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Abstract BACKGROUND: The genetic diversity of chemokines and chemokine receptors has been associated with the outcome of hepatitis B virus infection. The aim of this study was to evaluate whether the copy number variation in the CCL3L1 gene and the polymorphisms of CCR5Δ32 and CCR5-2459A→G (rs1799987) are associated with recurrent hepatitis B in liver transplantation for hepatitis B virus infection-related end- stage liver disease.
METHODS: A total of 185 transplant recipients were enrolled in this study. The genomic DNA was extracted from whole blood, the copy number of the CCL3L1 gene was determined by a quantitative real-time PCR based assay, CCR5Δ32 was detected by a sizing PCR method, and a single-nucleotide polymorphism in CCR5-2459 was detected by restriction fragment length polymorphism PCR.
RESULTS: No CCR5Δ32 mutation was detected in any of the individuals from China. Neither copy number variation nor polymorphism in CCR5-2459 was associated with post-transplant re-infection with hepatitis B virus. However, patients with fewer copies (<4) of the CCL3L1 gene compared with the population median in combination with the CCR5G allele had a significantly higher risk for recurrent hepatitis B (odds ratio=1.93, 95% CI: 1.00-3.69; P=0.047).
CONCLUSION: Patients possessing the compound decreased functional genotype of both CCL3L1 and CCR5 genes might be more likely to have recurrence of hepatitis B after transplantation.
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2011, Vol. 10 
