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Beneficial effects of splenectomy on liver regeneration in a rat model of massive hepatectomy |
Yan-Shun Ren, Nian-Song Qian, Yu Tang, Yong-Hui Liao, Wei-Hui Liu, Vikram Raut, Kai-Shan Tao and Ke-Feng Dou |
Xi an, China
Author Affiliations: Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi an 710032, China (Ren YS, Liao YH, Liu WH, Tao KS and Dou KF); Department of Hepatobiliary Surgery, PLA General Hospital, Beijing 100853, China (Qian NS); Department of Breast Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan (Qian NS and Raut V); Department of Ultrasound, PLA 302 Hospital, Beijing 100071, China (Tang Y)
Corresponding Author: Ke-Feng Dou, MD, Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi an 710032, China (Tel: 86-29-84771049; Fax: 86-29-84771049; Email: doukefengfmmu@gmail.com) |
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Abstract BACKGROUND: Small-for-size syndrome is a widely recognized clinical complication after living donor liver transplantation or extended hepatectomy due to inadequate liver mass. The purpose of this study was to investigate the role of splenectomy in rats after massive hepatectomy, a surrogate model of small-for-size graft.
METHODS: Rats were divided into eight groups, each with 20 animals: 50% hepatectomy (50% Hx), 50% hepatectomy+splenectomy (50% Hx+Sp), 60% Hx, 60% Hx+Sp, 70% Hx, 70% Hx+Sp, 90% Hx and 90% Hx+Sp. The following parameters were evaluated: liver function tests (ALT, AST and TBIL), liver regeneration ratio, DNA synthesis, proliferation cell nuclear antigen, hepatic oxygen delivery (HDO2) and hepatic oxygen consumption (HVO2).
RESULTS: The liver regeneration ratio was enhanced in the Hx+Sp groups (P<0.05). In addition, compared with the Hx groups, the Hx+Sp groups had better liver functions (P<0.05). DNA synthesis and proliferation cell nuclear antigen were also increased in the Hx+Sp groups compared with the Hx groups (P<0.05). Furthermore, in the Hx+Sp groups, HDO2 and HVO2 were increased over those in the Hx groups (P<0.05), and were positively correlated with the liver regeneration ratio.
CONCLUSIONS: Splenectomy significantly improved liver function, and enhanced DNA synthesis and proliferation cell nuclear antigen after massive hepatectomy in rats. This operation could be mediated through increased HDO2 and HVO2, which facilitate liver regeneration.
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