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| Hepatic ischemic preconditioning increases portal vein flow in experimental liver ischemia reperfusion injury |
| Estela RR Figueira, Joel A Rocha-Filho, Mauro Nakatani, Marcelo FS Buto, Eduardo R Tatebe, Vitor O Andre, Ivan Cecconello and Luiz AC D'Albuquerque |
São Paulo, Brazil
Author Affiliations: Department of Gastroenterology, Laboratory of Medical Investigations LIM37 Discipline of Liver and Gastrointestinal Transplantation (Figueira ERR, Cecconello I and D'Albuquerque LAC); Discipline of Anesthesiology (Rocha-Filho JA); School of Medicine, Medical Student and Scientific Research in Medicine FAPESP (Nakatani M, Buto MFS, Tatebe ER and Andre VO), Hospital das Clinicas, University of São Paulo, Brazil
Corresponding Author: Estela RR Figueira, MD, Department of Gastro¬enterology, University of São Paulo School of Medicine, Rua dos Ingleses 586, apt 194, 01329-000, São Paulo, SP, Brazil (Email: estelafigueira@me.com) |
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Abstract BACKGROUND: Ischemic preconditioning (IPC) has been shown to decrease liver injury and to increase hepatic microvascular perfusion after liver ischemia reperfusion. This study aimed to evaluate the effects of IPC on hemodynamics of the portal venous system.
METHODS: Thirty-two rats were randomized into two groups: IPC group and control group. The rats of the IPC group underwent IPC by 10 minutes of liver ischemia followed by 10 minutes of reperfusion before liver ischemia, and the rats of the control group were subjected to 60 minutes of partial liver ischemia. Non-ischemic lobes were resected immediately after reperfusion. The animals were studied at 4 hours and 12 hours after reperfusion. Mean arterial pressure, heart rate, portal vein flow and pressure were analyzed. Blood was collected for the determination of the levels of aspartate aminotransferase, alanine aminotransferase, calcium, lactate, pH, bicarbonate, and base excess.
RESULTS: IPC increased the mean portal vein flow at 4 hours and 12 hours after reperfusion. IPC recovered 78% of the mean portal vein flow at 12 hours after reperfusion. IPC decreased the levels of aspartate aminotransferase, alanine aminotransferase and lactate, and increased the levels of ionized calcium, bicarbonate and base excess at 12 hours after reperfusion.
CONCLUSIONS: This study demonstrated that IPC increases portal vein flow and enhances hepatoprotective effects in liver ischemia reperfusion. The better recovery of portal vein flow after IPC may be correlated with the lower levels of transaminases and with the better metabolic profile.
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2014, Vol. 13 
