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| Oncogenic role of microRNA-423-5p in hepatocellular carcinoma |
| Li-Ming Wu, Jian-Song Ji, Zhe Yang, Chun-Yang Xing, Ting-Ting Pan, Hai-Yang Xie, Feng Zhang, Li Zhuang, Lin Zhou and Shu-Sen Zheng |
Hangzhou, China
Author Affiliations: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China (Wu LM, Yang Z, Xing CY, Xie HY, Zhang F, Zhuang L, Zhou L and Zheng SS); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health; Key Laboratory of Organ Transplantation of Zhejiang Province; Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Wu LM, Yang Z, Xing CY, Pan TT, Xie HY, Zhang F, Zhuang L, Zhou L and Zheng SS); Lishui Hospital of Zhejiang University, Lishui 323000, China (Ji JS)
Corresponding Author: Shu-Sen Zheng, MD, PhD, FACS, Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Tel: 86-571- 87236570; Fax: 86-571-87236466; Email: shusenzheng@zju.edu.cn) |
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Abstract BACKGROUND: It has been found that microRNA-423-5p (miR423-5p) is an oncogenic factor and frequently upregulated in gastric carcinoma. However, the involvement of miR423-5p in hepatocellular carcinoma (HCC) has been rarely reported. The aim of this study was to assess whether miR423-5p is aberrantly expressed in HCC tissues, and to characterize its roles in the cancerous biology of HCC.
METHODS: HCC and corresponding nonmalignant tissues were obtained from 115 patients during liver transplantation to detect the expression level of miR423-5p. The miR423-5p mimic and inhibitor were transfected into LM3 cell line. Cell viability assay, cell cycle analysis, transwell invasion and migration experiments were used to evaluate the oncogenic role of miR423-5p.
RESULTS: miR423-5p was significantly upregulated in HCC compared with nonmalignant tissues, and this upregulation was negatively associated with recurrence-free survival. For patients beyond the Milan criteria, low expression of miR423-5p was correlated with better prognosis. Functional analysis showed that miR423-5p enhanced the proliferative, invasive and migratory capacity of HCC cells.
CONCLUSIONS: miR423-5p contributed to the tumorigenesis and progression of HCC. It could be a new predictor in HCC patients beyond the Milan criteria and would help to improve patient outcomes and enlarge recipient pools of liver transplantation.
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2015, Vol. 14 
