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| Silver-nanoparticle-coated biliary stent inhibits bacterial adhesion in bacterial cholangitis in swine |
| Wei Wen, Li-Mei Ma, Wei He, Xiao-Wei Tang, Yin Zhang, Xiang Wang, Li Liu and Zhi-Ning Fan |
Nanjing, China
Author Affiliations: Department of Digestive Endoscopy Center, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China (Wen W, Ma LM, Tang XW, Wang X, Liu L and Fan ZN); Nanjing Medical University, Nanjing 210029, China (Wen W); Department of Gastroenterology, The 81th Hospital of PLA, Nanjing 210002, China (Wen W); Department of Gastroenterology, Changzhou First People’s Hospital, Third Affiliated Hospital of Suzhou University, Changzhou 213003, China (He W and Zhang Y)
Corresponding Author: Zhi-Ning Fan, MD, PhD, Department of Digestive Endoscopy Center, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China (Tel/Fax: +86-25-58509931; Email: fanzhining@njmu.edu.cn) |
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Abstract BACKGROUND: One of the major limitations of biliary stents is the stent occlusion, which is closely related to the over-growth of bacteria. This study aimed to evaluate the feasibility of a novel silver-nanoparticle-coated polyurethane (Ag/PU) stent in bacterial cholangitis model in swine.
METHODS: Ag/PU was designed by coating silver nanoparticles on polyurethane (PU) stent. Twenty-four healthy pigs with bacterial cholangitis using Ag/PU and PU stents were randomly divided into an Ag/PU stent group (n=12) and a PU stent group (n=12), respectively. The stents were inserted by standard endoscopic retrograde cholangiopancreatography. Laboratory assay was performed for white blood cell (WBC) count, alanine aminotransferase (ALT), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) at baseline time, 8 hours, 1, 2, 3, and 7 days after stent placements. The segment of bile duct containing the stent was examined histologically ex vivo. Implanted biliary stents were examined by a scan electron microscope. The amount of silver release was also measured in vitro.
RESULTS: The number of inflammatory cells and level of ALT, IL-1β and TNF-α were significantly lower in the Ag/PU stent group than in the PU stent group. Hyperplasia of the mucosa was more severe in the PU stent group than in the Ag/PU stent group. In contrast to the biofilm of bacteria on the PU stent, fewer bacteria adhered to the Ag/PU stent.
CONCLUSIONS: PU biliary stents modified with silver nanoparticles are able to alleviate the inflammation of pigs with bacterial cholangitis. Silver-nanoparticle-coated stents are resistant to bacterial adhesion.
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2016, Vol. 15 
