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| Role of phosphorylated Smad3 signal components in intraductal papillary mucinous neoplasm of pancreas ? |
| Yuichi Hori a , Tsukasa Ikeura a , Takashi Yamaguchi a , Katsunori Yoshida a , Koichi Matsuzaki a , Mitsuaki Ishida b , Sohei Satoi c , Kazuichi Okazaki a , ∗ |
a Department of Gastroenterology and Hepatology, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 5731191, Japan
b Department of Pathology, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 5731191, Japan
c Department of Surgery, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 5731191, Japan
? Note: This work has been published in the form of an abstract for the Joint 50th
Anniversary Meeting of the American Pancreatic Association and Japan Pancreas Society,
November 6–9, 2019, Maui, Hawaii, USA.
∗ Corresponding author.
E-mail address: okazaki@hirakata.kmu.ac.jp (K. Okazaki). |
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Abstract Background: Malignant intraductal papillary mucinous neoplasm (IPMN) has poor prognosis. The carcinogenesis of IPMN is not clear. The aim of this study was to clarify transitions in phosphorylated Smad3 signaling during IPMN carcinogenesis.
Methods: By using immunohistochemistry, we examined the expression of pSmad3C and pSmad3L from 51 IPMN surgical specimens resected at our institution between 2010 and 2013. We also examined the expression of Ki-67, c-Myc and p-JNK.
Results: The median immunostaining index of pSmad3C was 79.2% in low-grade dysplasia, 74.9% in high-grade dysplasia, and 42.0% in invasive carcinoma ( P < 0.01), whereas that of pSmad3L was 3.4%, 4.3%, and 42.4%, respectively ( P < 0.01). There was a negative relationship between the expression of pS- mad3C and c-Myc ( P < 0.001, r = -0.615) and a positive relationship between the expression of pSmad3L and c-Myc ( P < 0.001, r = 0.696). Negative relationship between the expression of pSmad3C and Ki-67 ( P < 0.01, r = -0.610) and positive relationship between the expression of pSmad3L and Ki-67 ( P < 0.01, r = 0.731) were confirmed. p-JNK-positive cells were frequently observed among pSmad3L-positive cancer cells. The median of pSmad3L/pSmad3C ratio in the non-recurrence group and the recurrence group were 0.58 (range, 0.05–0.93), 3.83 (range, 0.85–5.96), respectively ( P = 0.02). The median immunostaining index of c-Myc in the non-recurrence group and the recurrence group were 2.91 (range, 0–36.9) and 82.1 (range, 46.2–97.1), respectively ( P = 0.02). The median immunostaining index of Ki-67 in the non-recurrence group and the recurrence group were 12.9 (range 5.7–30.8) and 90.9 (range 52.9–98.5), respectively ( P = 0.02).
Conclusions: pSmad3L was upregulated in malignant IPMN. pSmad3L/pSmad3C ratio may be a useful prognostic factor in IPMN.
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2020, Vol. 19 
