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Exosome is involved in liver graft protection after remote ischemia reperfusion conditioning |
Jian-Hui Li a , b , # , Jun-Jun Jia c , # , Ning He c , Xue-Lian Zhou d , Yin-Biao Qiao c , Hai-Yang Xie b , Lin Zhou b , Shu-Sen Zheng a , b , c , ∗ |
a Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou 310022, China
b NHC Key Laboratory of Combined Multi-organ Transplantation, Hangzhou 310022, China
c Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
d Department of Endocrinology, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China
∗Corresponding author at: Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou, 310022, China
E-mail address: shusenzheng@zju.edu.cn (S.-S. Zheng) .
# Contributed equally. |
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Abstract Background: Remote ischemic perconditioning (RIPerC) has been demonstrated to protect grafts from hepatic ischemia-reperfusion injury (IRI). This study investigated the role of exosomes in RIPerC of liver grafts in rats.
Methods: Twenty-five rats (including 10 donors) were randomly divided into five groups ( n = 5 each group): five rats were used as sham-operated controls (Sham), ten rats were for orthotopic liver transplantation (OLT, 5 donors and 5 recipients) and ten rats were for OLT + RIPerC (5 donors and 5 recipients). Liver architecture and function were evaluated.
Results: Compared to the OLT group, the OLT + RIPerC group exhibited significantly improved liver graft histopathology and liver function ( P < 0.05). Furthermore, the number of exosomes and the level of P-Akt were increased in the OLT + RIPerC group.
Conclusions: RIPerC effectively improves graft architecture and function, and this protective effect may be related to the increased number of exosomes. The upregulation of P-Akt may be involved in underlying mechanisms.
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