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| The role of inflammatory mediators in severe acute pancreatitis and regulation of glucocorticoids |
| Zi-Fa Wang, Cheng-En Pan, Yi Lu, Shao-Gao Liu, Guan-Jun Zhang and Xue-Bin Zhang |
Xi’an, China
From the Departments of Surgery (Wang ZF, Pan CE, Lu Y and Liu SG); and Pathology (Zhang GJ and Zhang XB), First Hospital, Xi’an Jiaotong University, Xi’an 710061, China; and the Department of Surgery, University of Pittsburgh, NW607, Montefiore University Hospital, 3459 Fifth Avenue, Pittsburgh, PA 15213, USA (Wang ZF)
Correspondence: Zi-Fa Wang, MD, PhD, Department of Surgery, University of Pittsburgh, NW607, Montefiore University Hospital, 3459 Fifth Avenue, Pittsburgh, PA15213, USA (Tel: 1-412-647-5696; Fax: 1-412-647-5959; Email: ziw3@pitt.edu) |
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Abstract OBJECTIVE: To investigate the effect of glucocorticoids on systemic inflammatory mediator release in rats with acute pancreatitis and the outcome of dexamethasone in treatment of acute pancreatitis.
METHODS: Sixty-eight Wistar rats were divided into sham, acute pancreatitis, and treatment (intravenous dexamethasone 0.5 mg/kg) groups. Experimental acute pancreatitis was induced by the injection of 5% sodium taurocholate (0.1 ml/100 mg body weight) into the pancreatic-biliary duct. The blood samples were obtained and examined for 6-keto-PGI1α, TXB2 and IL-6 postoperatively at 3, 6 and 12 hours, respectively. The pancreatic samples were evaluated by a blinded method. Twelve-hour survival rate was determined and compared between the groups.
RESULTS: The high serum concentrations of 6-keto-PGI1α, TXB2 and IL-6 were noted in the rats with acute pancreatitis associated with pancreatic hemorrhage and necrosis. Their 12-hour survival rate was 42.9%. The rats in the treatment group survived with significantly reduced serum concentrations of 6-keto-PGI1α, TXB2 and IL-6 (P<0.05). Their pancreatic morphology was normal.
CONCLUSION: Dexamethasone may reduce the serum concentration of 6-keto-PGI1α, TXB2, and IL-6, and the severity of acute pancreatitis while increasing the survival rate of rats with acute pancreatitis.
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2003, Vol. 2 
