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| Micro-positron emission tomography imaging of angiogenesis based on (18)F-RGD for assessing liver metastasis of colorectal cancer |
| Ming-Yu Zhang a , # , Hui-Jie Jiang b , # , Hao Jiang b , Rong-Jun Zhang c , Zhen-Chang Wang d , ∗ |
a Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
b Department of Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China
c Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China
d Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
∗ Corresponding author.
E-mail address: cjr.wzhch@vip.163.com (Z.-C. Wang).
# Contributed equally. |
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Abstract Background: Positron emission tomography (PET) imaging is a non-invasive method to visualize and quantify the tumor microenvironment. This study aimed to explore the feasibility of 18 F-AIF-NOTA- E[PEG 4 -c(RGDfk)] 2 (denoted as 18 F-RGD) PET quantitative parameters to distinguish the angiogenesis in colorectal cancer (CRC) mice which has different metastatic potential.
Methods: Twenty LoVo and twenty LS174T of CRC liver metastases animal models were established by implantation of human CRC cell lines via intrasplenic injection. Radiotracer-based micro-PET imaging of animal model was performed and the uptake of 18 F-RGD tracer in the tumor tissues was quantified as tumor-to-liver maximum or mean standardized uptake value (SUVmax or SUVmean) ratio. Pearson correlation was used to analyze the relationship between radioactive parameters and tumor markers.
Results: The SUVmax and SUVmean ratios of LoVo model were significantly higher than those of LS174T in both liver metastasis and primary tumor lesions (P < 0.05). A significant difference was observed in both vascular endothelial growth factor (VEGF) and Ki67 expressions between LoVo and LS174T primary tumors (P < 0.05). The tumor-to-liver SUVmax or SUVmean ratio of 18 F-RGD showed a moderate correlation with VEGF expression (r = 0.5700, P = 0.001 and r = 0.6657, P < 0.001, respectively), but the SUVmean ration showed a weak correlation with Ki67 expression (r = 0.3706, P < 0.05). The areas under the receiver operating characteristic (ROC) curves of 18 F-RGD SUVmean ratio, SUVmax ratio for differentiating LoVo from LS174T tumor were 0.801 and 0.759, respectively.
Conclusions: The tumor-to-liver SUVmean ratio of 18 F-RGD was a promising image parameter for the process of monitoring tumor angiogenesis in CRC xenograft mice model.
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2021, Vol. 20 
