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| The effect of mycophenolate acid on hepatitis B virus replication in vitro |
| Jian Wu, Hai-Yang Xie, Guo-Ping Jiang, Xiao Xu and Shu-Sen Zheng |
Hangzhou, China
From the Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Wu J, Xie HY, Jiang GP, Xu X and Zheng SS)
Correspondence: Shu-Sen Zheng, MD, PhD, Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Tel: 86-571-87236567; Fax: 86-571-87236570; Email: zhengss@mail.hz.zj.cn) |
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Abstract OBJECTIVE: To use 2.2.15 cell line to determine the effects of mycophenolate acid (MPA) on hepatitis B virus (HBV) replication and viral protein synthesis in vitro.
METHODS: The 2.2.15 cells were treated with different concentration of MPA (1-50 μg/ml) for 12 days. HBsAg and HBeAg were detected in the supernatant fluid by ELISA and intracellular HBV DNA was analyzed quantitatively by slot blot hybridization.
RESULTS: MPA could suppress the expression of HBsAg and HBeAg, and the higher concentration of MPA induced lower expression of HBsAg and HBeAg. The suppression rates of MPA for HBsAg and HBeAg at a concentration of 50 μg/ml were 34.2% and 24.1% respectively. The expression of HBV DNA was only 49% as compared with controls when treated with MPA at a concentration of 50 μg/ml.
CONCLUSIONS: Mycophenolate acid can suppress the expression of HBsAg and HBeAg as well as the replication of HBV DNA in the 2.2.15 cell. The suppressive degree is dose-dependent.
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2003, Vol. 2 
