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| Analysis of gene expression profiles in pancreatic carcinoma by using cDNA microarray |
| Xian-Jun Yu, Jiang Long, De-Liang Fu, Qun-Hua Zhang and Quan-Xin Ni |
Shanghai, China
From the Center for Pancreatic Cancer, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China (Yu XJ, Long J, Fu DL, Zhang QH and Ni QX)
Correspondence: Xian-Jun Yu, MD, Center for Pancreatic Cancer, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China (Tel: 86-21-62489999ext6425; Fax: 86-21-62483696; Email: yuxianjun@hotmail.com) |
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Abstract OBJECTIVES: To survey the gene expression profiles in pancreatic carcinoma by using cDNA microarray and detect target genes for further study.
METHODS: Three mixed samples from 2 cases of normal pancreatic tissue and 4 cases of moderate-differentiated pancreatic carcinoma were studied by means of cDNA microarray consisting of 18 000 genes.
RESULTS: 1484 and 1353 different expressed genes were observed in two cancer samples respectively. We identified 455 genes altered with the same tendency in both samples, including 102 up-regulated and 353 down-regulated genes. There were 274 known genes and 181 unknown genes; 27.8% and 52.0% genes respectively had an expression level in cancer that was 2-fold higher or lower than that in normal samples. Tumor suppressor genes, growth factors and receptor genes, signal conduction genes, transcription factor genes were identified.
CONCLUSIONS: cDNA microarray is an efficient and high-throughout method to investigate gene expression profiles in pancreatic carcinoma. MBD1, EDG1 and gene hypermethylation mechanism would play an important role in the pathogenesis of pancreatic carcinoma.
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2003, Vol. 2 
